Professor Tuomas Knowles

Professor of Physical Chemistry and Biophysics

1920 Professor of Physical Chemistry

Our research

We study the physical and chemical aspects of the behaviour of biopolymers and other soft systems. Much of our work has been focused on the physical aspects underlying the self-assembly of protein molecules. Self-organisation is the driving force generating complex matter in nature, and the process by which the machinery providing functionality in living systems is assembled. The goal of our research is to understand the physical and chemical factors which control the structures and dynamics of biomolecular assemblies, and the connections between the nanoscale characteristics of the component molecules and the physical properties of large-scale assemblies and their behaviour on a mesoscopic to macroscopic scale. The techniques used in our laboratory include biosensors, optical lithography, microfluidic devices and scanning probe microscopy and spectroscopy. We work both with natural and synthetic polymers and our interests range from fundamental chemical physics to technological applications in material science and molecular medicine.

Watch Professor Knowles discuss his research

Take a tour of the Sir Rodney Sweetnam laboratory

Publications

Quantitative sensing of microviscosity in protocells and amyloid materials using fluorescence lifetime imaging of molecular rotors

AJ Thompson, T-YD Tang, TW Herling, CRC Hak, S Mann, TPJ Knowles, MK Kuimova

Progress in Biomedical Optics and Imaging Proceedings of SPIE

(2014)

8947

89471C

(doi: 10.1117/12.2037851)

Chemical kinetics for drug discovery to combat protein aggregation diseases

P Arosio, M Vendruscolo, CM Dobson, TPJ Knowles

Trends in Pharmacological Sciences

(2014)

127

(doi: 10.1016/j.tips.2013.12.005)

The role of stable α-synuclein oligomers in the molecular events underlying amyloid formation.

N Lorenzen, SB Nielsen, AK Buell, JD Kaspersen, P Arosio, BS Vad, W Paslawski, G Christiansen, Z Valnickova-Hansen, M Andreasen, JJ Enghild, JS Pedersen, CM Dobson, TPJ Knowles, DE Otzen

Journal of the American Chemical Society

(2014)

136

3859

(doi: 10.1021/ja411577t)

Single point mutations induce a switch in the molecular mechanism of the aggregation of the Alzheimer's disease associated Aβ42 peptide

B Bolognesi, SIA Cohen, P Aran Terol, EK Esbjörner, S Giorgetti, MF Mossuto, A Natalello, AC Brorsson, TPJ Knowles, CM Dobson, LM Luheshi

ACS Chemical Biology

(2014)

378

(doi: 10.1021/cb400616y)

Diffuse transition state structure for the unfolding of a leucine-rich repeat protein

SE Kelly, G Meisl, PJE Rowling, SH McLaughlin, T Knowles, LS Itzhaki

Phys Chem Chem Phys

(2014)

6448

(doi: 10.1039/c3cp54818j)

Targeting the Intrinsically Disordered Structural Ensemble of α-Synuclein by Small Molecules as a Potential Therapeutic Strategy for Parkinson's Disease

G Tóth, SJ Gardai, W Zago, CW Bertoncini, N Cremades, SL Roy, MA Tambe, J-C Rochet, C Galvagnion, G Skibinski, S Finkbeiner, M Bova, K Regnstrom, S-S Chiou, J Johnston, K Callaway, JP Anderson, MF Jobling, AK Buell, TA Yednock, TPJ Knowles, M Vendruscolo, J Christodoulou, CM Dobson, D Schenk, L McConlogue

PloS one

(2014)

e87133

(doi: 10.1371/journal.pone.0087133)