Sir Henry Wellcome Research Fellow
My main research interests lie at the interface of experimental and theoretical techniques to obtain atomic-resolution understanding of the structure and dynamics of nucleo-protein complexes. During my Ph. D., I studied the dynamics of the HIV-1 TAR RNA using NMR spectroscopy data in combination with molecular dynamics simulations. For my postdoctoral research, I have now taken up the long-standing challenge of determining the structure of the whole HIV-1 ribonucleoprotein transactivation complex (TAC) by X-ray crystallography.
Publications
Simultaneous NMR characterisation of multiple minima in the free energy landscape of an RNA UUCG tetraloop
– Physical chemistry chemical physics : PCCP
(2017)
19,
2797
(doi: 10.1039/c6cp08313g)
Structure of a low-population binding intermediate in protein-RNA recognition
– Proceedings of the National Academy of Sciences of the United States of America
(2016)
113,
7171
(doi: 10.1073/pnas.1521349113)
Constructing free energy landscapes of RNAs at atomic resolution and characterisation of their excited states
– Journal of Biomolecular Structure and Dynamics
(2015)
33,
56
A method of determining RNA conformational ensembles using structure-based calculations of residual dipolar couplings.
– Journal of Chemical Physics
(2013)
138,
215103
(doi: 10.1063/1.4804301)
Facile backbone (1H, 15N, 13Ca, and 13C') assignment of 13C/15N-labeled proteins using orthogonal projection planes of HNN and HN(C)N experiments and its automation.
– Magnetic resonance in chemistry : MRC
(2012)
50,
357
(doi: 10.1002/mrc.3801)
Denaturation of hiv-1 protease (pr) monomer by acetic acid: Mechanistic and trajectory insights from molecular dynamics simulations and nmr
– Journal of biomolecular structure & dynamics
(2012)
29,
893
(doi: 10.1080/073911012010525025)
Visualization of early events in acetic acid denaturation of HIV-1 Protease: A molecular dynamics study
– PLoS One
(2011)
6,
e19830
(doi: 10.1371/journal.pone.0019830)
AUTOBA: Automation of backbone assignment from HN(C)N suite of experiments
– J Biomol NMR
(2011)
50,
285
(doi: 10.1007/s10858-011-9518-0)
Structure and dynamics of double helical dna in torsion angle hyperspace: A molecular mechanics approach
– Journal of biomolecular structure & dynamics
(2010)
27,
695